Journal: Biomaterials Research
Article Title: Improvement of Charcot-Marie-Tooth Phenotype with a Nanocomplex Treatment in Two Transgenic Models of CMT1A
doi: 10.34133/bmr.0009
Figure Lengend Snippet: Investigation of the anti-inflammatory effect of NanoCur in CMT1A. (A) Anti-CD68 [ED1] staining of sciatic nerves using 3,3-diaminobenzidine staining (IHC) for macrophage detection in sciatic nerve sections of WT-NT, CMT1A-NT, and CMT1A-NanoCur rats, in addition to the quantification of the percentage area of macrophages present in the nerve sections. Representative figures along with barograms of the quantification of the average protein expression level of (B) Macrophage Migration Inhibition Factor MIF and (C) myeloperoxidase with respect to the total protein. (D) Stain-free gel of the representative figure for MIF (Gel 1) and myeloperoxidase (Gel 2) (E) Representative figure of the fluorescent staining of mouse sciatic nerves of WT, Tg +/- , Tg +/- -T, Tg +/+ , and Tg +/+ -T with the macrophage marker F4/80. Results are represented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA test followed by post hoc Tukey test; *: P < 0.05.
Article Snippet: The sciatic nerves of rats were stained with rat anti-CD68 (ED1) primary antibody (Biorad, MCA341, 1:1,000) using the avidin-biotin 3,3-diaminobenzidine peroxidase kit (Vectastain; Vector Laboratories) in accordance with the supplier’s protocol and counterstained with Mayer’s hematoxylin, then observed using a Nikon optical microscope.
Techniques: Staining, Expressing, Migration, Inhibition, Marker
Bio-Rad is a verified supplier 
![Investigation of the anti-inflammatory effect of NanoCur in CMT1A. (A) <t>Anti-CD68</t> <t>[ED1]</t> staining of sciatic nerves using 3,3-diaminobenzidine staining (IHC) for macrophage detection in sciatic nerve sections of WT-NT, CMT1A-NT, and CMT1A-NanoCur rats, in addition to the quantification of the percentage area of macrophages present in the nerve sections. Representative figures along with barograms of the quantification of the average protein expression level of (B) Macrophage Migration Inhibition Factor MIF and (C) myeloperoxidase with respect to the total protein. (D) Stain-free gel of the representative figure for MIF (Gel 1) and myeloperoxidase (Gel 2) (E) Representative figure of the fluorescent staining of mouse sciatic nerves of WT, Tg +/- , Tg +/- -T, Tg +/+ , and Tg +/+ -T with the macrophage marker F4/80. Results are represented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA test followed by post hoc Tukey test; *: P < 0.05.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1932/pmc10981932/pmc10981932__bmr.0009.fig.005.jpg)