anti ed1  (Bio-Rad)

Journal: bioRxiv

Article Title: The SRG RAT® supports human cell xenotransplantation through enhanced tumor microenvironment interactions

doi: 10.1101/2025.03.27.645250

Figure Lengend Snippet: All slides were obtained serially from the section used for Xenium analysis A , Iba-1 immunolabeling of macrophages by DAB chromogen in mouse (left) and rat (right) tumors. Macrophages adjacent areas of necrosis (black arrowhead), in areas of abundant stroma (black arrows) and less stroma (open arrows) are shown. Areas of necrosis (N), capsule (C), denser stroma (*) are indicated. Scale bar indicates 2 mm (4x; top row) and 1 mm, 10x; bottom row). Quantification can be found in Supplemental Figure 12. B , Iba-1 immunolabeling of macrophages by DAB chromogen in stroma-dense (left) or stroma-poor (right) regions of tumors taken from the SRG rat. Scale bar indicates 500 μm (10x). C, CD68/CD163 dual IF for macrophages in stroma-dense and stroma-poor regions of SRG rat. DAPI: top row, CD68 (green channel), CD163 (red channel), Merged image: bottom row; CD68+CD163+ cells are yellow. Scale bar indicates 200 μm (30x).

Article Snippet: Primary antibody incubation (CD68: 1:300, mouse mAb [ED1], Novus Biologicals #NB600-985; CD163: 1:500, rabbit mAb [EPR19518], Abcam #ab182422) was performed at room temperature for 30 minutes.

Techniques: Immunolabeling

Journal: Biomaterials Research

Article Title: Improvement of Charcot-Marie-Tooth Phenotype with a Nanocomplex Treatment in Two Transgenic Models of CMT1A

doi: 10.34133/bmr.0009

Figure Lengend Snippet: Investigation of the anti-inflammatory effect of NanoCur in CMT1A. (A) Anti-CD68 [ED1] staining of sciatic nerves using 3,3-diaminobenzidine staining (IHC) for macrophage detection in sciatic nerve sections of WT-NT, CMT1A-NT, and CMT1A-NanoCur rats, in addition to the quantification of the percentage area of macrophages present in the nerve sections. Representative figures along with barograms of the quantification of the average protein expression level of (B) Macrophage Migration Inhibition Factor MIF and (C) myeloperoxidase with respect to the total protein. (D) Stain-free gel of the representative figure for MIF (Gel 1) and myeloperoxidase (Gel 2) (E) Representative figure of the fluorescent staining of mouse sciatic nerves of WT, Tg +/- , Tg +/- -T, Tg +/+ , and Tg +/+ -T with the macrophage marker F4/80. Results are represented as mean ± SEM. Statistical analysis was performed using 1-way ANOVA test followed by post hoc Tukey test; *: P < 0.05.

Article Snippet: The sciatic nerves of rats were stained with rat anti-CD68 (ED1) primary antibody (Biorad, MCA341, 1:1,000) using the avidin-biotin 3,3-diaminobenzidine peroxidase kit (Vectastain; Vector Laboratories) in accordance with the supplier’s protocol and counterstained with Mayer’s hematoxylin, then observed using a Nikon optical microscope.

Techniques: Staining, Expressing, Migration, Inhibition, Marker